Insights into carbon loss reduction during aerobic composting of organic solid waste: A meta-analysis and comprehensive literature review.

Carbon neutrality is now receiving global concerns for the sustainable development of human societies, of which how to reduce greenhouse gases (GHGs) emissions and enhance carbon conservation and sequestration becomes increasingly critical. Therefore, this study conducted a meta-analysis and literature review to assess carbon loss and to explore the main factors that impact carbon loss during organic solid waste (OSW) composting. The results indicated that over 40 % of carbon was lost through composting, mainly as CO2-C and merely as CH4-C. Experimental scale, feedstock varieties, composting systems, etc., all impacted the carbon loss, and there was generally higher carbon loss under optimal conditions (i.e., C/N ratio (15-25), pH (6.5-7.5), moisture content (65-75 %)). Most mitigation strategies in conventional composting (CC) systems (e.g., additive supplementary, feedstock adjustment, and optimized aeration, etc.) barely mediated the TC and CO2-C loss but dramatically reduced the emission of CH4-C through composting. Among them, feedstock adjustment by elevating the feedstock C/N ratio effectively reduced the TC loss, and chemical additives facilitated the conservation of both carbon and nitrogen. By comparison, there was generally higher carbon loss in the novel composting systems (e.g. hyperthermophilic and electric field enhanced composting, etc.). However, the impacts of different mitigation strategies and novel composting systems on carbon loss reduction through composting were probably underestimated for the inappropriate evaluation methods (composting period-dependent instead of maturity originated). Therefore, further studies are needed to explore carbon transformation through composting, to establish methods and standards for carbon loss evaluation, and to develop novel techniques and systems for enhanced carbon conservation through composting. Overall, the results of this study could provide a reference for carbon-friendly composting for future OSW management under the background of global carbon neutrality.

LINC01224 facilitates the proliferation and inhibits the radiosensitivity of melanoma cells through the miR-193a-5p/NR1D2 axis.

Melanoma is a skin cancer characterized by early metastasis and high mortality. Radiotherapy is a common treatment for melanoma in patients. Long noncoding RNAs play pivotal roles in regulating the radiosensitivity of many tumors, including melanomas. In this study, the role of LINC01224 in the radiosensitivity of melanoma cells was explored. The expression of LINC01224 in melanoma was examined by reverse transcription-quantitative polymerase chain reaction, and the results showed that LINC01224 was upregulated in melanoma tissues and cells. The effects of LINC01224 on cell proliferation and apoptosis in melanoma were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), colony formation, and flow cytometry assays. The effects of LINC01224 on the radiosensitivity of melanoma were analyzed by colony formation assay. The results implied that LINC01224 knockdown inhibited cell viability and proliferation but enhanced cell apoptosis and radiosensitivity. Luciferase reporter and RNA pull-down assays were performed to evaluate the relationships between LINC01224 and miR-193a-5p or miR-193a-5p and nuclear receptor subfamily 1 group D member 2 (NR1D2). We found that LINC01224 binds to miR-193a-5p, which directly targets NR1D2. In addition, we discovered that LINC01224 upregulated NR1D2 expression by sponging miR-193a-5p in melanoma cells. Overall, the data collected in this study suggest that LINC01224 exerts oncogenic effects in melanoma via the miR-193a-5p/NR1D2 axis.

Hydrothermal conversion of Chinese cabbage residue for sustainable agriculture: Influence of process parameters on hydrochar and hydrolysate.

The demands on novel and sustainable techniques for vegetable waste (VW) valorization continues to increase during the past few decades due to the growing waste production under the flourishing vegetable industries. In this study, Chinese cabbage residues were hydrothermal carbonization (HTC) at 180, 200, 220 and 240 °C for 2 to 6 h to explore the impacts of process parameters on the characteristics of hydrochars and hydrolysates and their feasibility in sustainable agriculture. Results indicated that hydrothermal temperature had a greater impact on cabbage residue hydrolysis than the residence time. With the rising reaction severity, hydrochars became more alkaline with higher amount of ash and carbon (C), while the pH and dissolved organic nitrogen (DON) and NH4+-N in the hydrolysate were gradually reduced. The thermogravimetric analysis (TG-DTG) indicated that organic constitutions in the feedstock went through incomplete decomposition. Although the recalcitrance index (R50) steadily increased through HTC (0.37-0.46), hydrochars were unstable and would not applicable for carbon sequestration. Furthermore, hydrochars and hydrolysate would be optimal media for plants seedling and growth for the abundant nutrients and dissolved organic compounds but reduced phytotoxicity. In conclusion, these results showed that HTC is highly applicable for vegetable waste management for sustainable agriculture.

Polyphenol-Enriched Extracts from Trapa acornis Husks Inhibit Her2-Positive SK-BR-3 Breast Cancer Cell Proliferation and In Vivo Tumor Angiogenesis.

The study aimed to investigate the antitumor effects of Trapa acornis husks (TAH) extract on SK-BR-3 cells of Her2-positive breast cancer. The bioactive compounds of TAH extracts were analyzed qualitatively and quantitatively by Ultra-Performance Liquid Chromatography/Mass Spectrometry (UPLC-MS)/high-performance liquid chromatographic system (HPLC). The effects of TAH extracts on cell proliferation, cell cycle, and apoptosis of SK-BR-3 cells were determined by CCK-8 and flow cytometry. Besides, the In Vivo antitumor effect of TAH extracts was detected. UPLC-MS/HPLC showed that the main bioactive compounds of TAH were gallic acid and galloylglucose derivatives. TAH extracts significantly inhibited the proliferation of SK-BR-3 cells in a dose- and time-dependent manner (P < 0.01). With the increase of TAH extracts concentration, cells in G2/M stage were increased and cell apoptosis was significantly increased. Immunohistochemical analysis showed that TAH extracts can significantly reduce the positive expression rate of Ki67 and Factor VIII index in tumor tissues. The mRNA expression levels of VEGF, MMP2, MMP9, and uPA were reduced after TAH extracts intervention (P < 0.01). TAH extracts also decreased the protein expression of p-Her2, p-ERK1/2, VEGF, MMP2, MMP9, and uPA (P < 0.01). In conclusion, polyphenol-enriched extracts from TAH might inhibit breast cancer cell proliferation and In Vivo tumor angiogenesis.

Bioremediation of Cd-spiked soil using earthworms (Eisenia fetida): Enhancement with biochar and Bacillus megatherium application.

In this study, we evaluated the influence of biochar and Bacillus megatherium on Cd removal from artificially contaminated soils using earthworms (Eisenia fetida). Within a 35-days remediation period, over 30% of Cd was removed by earthworms from the contaminated soil (with Cd at âˆ¼ 2.5 mg kg-1), and both additives facilitated Cd removal. Additionally, over 22% reduction in the extractable Cd contents was also achieved by earthworms. Cd accumulated in earthworms steadily increased through remediation, and the accumulated Cd decreased in the order of earthworm + biochar (T3) > earthworm + Bacillus megatherium (T4) > earthworm alone (T2). The bioaccumulation factors (BCF) were above 1, indicating the enrichment of Cd in earthworms, and there were higher BCF for both T4 (944%) and T3 (845%). The ingestion of metal-bonded biochar particle and the elevated Cd mobility would be the main reason for the enhanced Cd-remediation by earthworms under T3 and T4, respectively. Through remediation, microbiota communities in both, soil and earthworm guts, demonstrated high similarity, while a lower level of bacterial abundance was observed in earthworm guts compared with that in soils. Eventually, soils became more fertile and demonstrated higher enzyme activities after remediation. Therefore, we concluded that earthworm, alone or combined with biochar or Bacillus megatherium could be an alternative method for Cd-contaminated soil remediation.